Mouse IgM ELISA Kit Plate

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Product Detail

Standard - EK276.jpg

BrandMultiSciences
CatNum70-EK276
ProductNameMouse IgM ELISA Kit
CustomsNameMouse IgM ELISA Kit
ApplicationELISA
ReactivityMouse
Assay TypeSandwich ELISA
Suitable Sample Typeserum, plasma, cell culture supernates
Format96-well strip plate
Storage4? (unopened) standard stored at -20?, others stored at 4? (opened)
Shipping Condition4?
Sample Volume10 µl (prediluted)
Sensitivity19.26 pg/ml
Standard Curve Range0.50 - 32 ng/ml
Spike Recovery Range----
Mean Spike Recovery----
CV of Intra plate3.2 % - 4.3 %
CV of Inter plate2.9 % - 7.6 %
Components96-well polystyrene microplate coated with a monoclonal antibody against IgM
Mouse IgM Standard, lyophilized
IgM Detect Antibody
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
DescribtionThis assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of mouse IgM. The Mouse IgM ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Immunoglobulin M (IgM) is a basic antibody that is produced by B cells. It is the first antibody to
appear in response to initial exposure to an antigen. The spleen, where plasmablasts responsible for antibody production reside, is the major site of specific IgM production. IgM in normal serum is often found to bind to specific antigens, even in the absence of prior immunization. For this reason IgM has sometimes been called a "natural antibody". Demonstrating IgM antibodies in a patient's serum indicates recent infection, or in a neonate's serum indicates intrauterine infection.
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